| MYDGF

Myeloid-Derived Growth Factor(MYDGF),is a Bone marrow-derived monocyte protein, and it is correlated with enhanced metabolic activity,suppression of apoptosis, and stimulation of cell proliferation. Involved in positive regulation of angiogenesis and positive regulation of endothelial cell proliferation. Located in Golgi apparatus; endoplasmic reticulum; and extracellular space.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	1000	500	250	125	62.5	31.2	15.6	0
OD	2.15	1.63	1.13	0.71	0.5	0.33	0.23	0.07
Corrected OD	2.08	1.56	1.06	0.64	0.43	0.26	0.16	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse MYDGF spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Mouse MYDGF and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-97	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	88-103	87-108	105-115
	Average (%)	94	98	108